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The polymerase chain reaction(PCR)method was used to determine the presence and amount of human T lymphotropic virus type I(HTLV-I)proviral DNA in central nervous system(CNS)tissue obtained at autopsy from 6 patients with HTLV-I-associated myelopathy(HAM)/tropical spastic paraparesis(TSP),1 patient with adult T-cell leukemia(ATL)and CNS infiltration of leukemic cells,and 9 control subjects with other neurological disorders.HTLV-I pX and env but not pol DNA were detected in CNS tissue from 5 of 6 patients with HAM/TSP.The ATL samples were positive for pX,env,and pol DNA by PCR.None of the control samples was consistently positive for HTLV-I by PCR,but all showed positive bands on B-globin PCR. Quantitative PCR combined with histological studies showed no correlation between HTLV-I proviral DNA amounts and extent of perivascular mononuclear cell infiltration in the HAM/TSP CNS.Also,the amounts of pX and probably env DNA were greater in the HAM/TSP samples than in the ATL sample, although the extent of mononuclear cell infiltration was far less in the HAM/TSP samples than in the ATL sample.Therefore,in addition to infiltrating mononuclear cells,constituent cells of the CNS may harbor the HTLV-I genome,at least in the pX and env regions,in patients with HAM/TSP. |
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human T-lymphotropic virus type I(HTLV-I)
myelopathy
paraparesis
paraparesis,spastic
paraparesis,spastic,tropical
polymerase chain reaction
viral infection,CNS
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